broad range polypeptide marker (Thermo Fisher)
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Thermo Fisher
broad range polypeptide marker
Broad Range Polypeptide Marker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/broad+range+polypeptide+marker/broad+range+polypeptide+marker/pm37229811-136-23-27
Average 90 stars, based on 1 article reviews
Broad Range Polypeptide Marker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/broad+range+polypeptide+marker/broad+range+polypeptide+marker/pm37229811-136-23-27
Average 90 stars, based on 1 article reviews
broad range polypeptide marker - by Bioz Stars,
2026-09
90/100 stars
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Western Blot:Article Title: LAT1 enables T cell activation under inflammatory conditions. Article Snippet: The aim of this study was to assess the L-type amino acid transporter-1 (LAT1) as a possible therapeutic target for rheumatoid arthritis (RA).. Synovial LAT1 expression in RA was monitored by immunohistochemistry and transcriptomic datasets.. The contribution of LAT1 to gene expression and immune synapse formation was assessed by RNA-sequencing and total internal reflection fluorescent (TIRF) microscopy, respectively. Article Title: LAT1 enables T cell activation under inflammatory conditions: a new therapeutic target for rheumatoid arthritis Article Snippet: Lysates were clarified by centrifugation, and the protein content was determined using Pierce BCA protein assay kit (Thermo Fisher) according to the manufacturer’s recommendation. .. For immunoblots, equal amounts of each protein sample were separated on a NuPAGE Novex 4-12% Bis-Tris gel followed by conventional blotting and a Article Title: Inhibition of Sec61-dependent translocation by mycolactone uncouples the integrated stress response from ER stress, driving cytotoxicity via translational activation of ATF4 Article Snippet: For western blots, cells were lysed either in RIPA buffer (Sigma) supplemented with protease inhibitors (Sigma) and phosphatase inhibitors (1 mM sodium pyrophosphate, 1 mM PMSF, 5 mM sodium fluoride and 1.75 mM β-glycerophosphate) or gel sample buffer followed by sonication for 30 s. Following RIPA lysis, lysates were clarified by centrifugation, and the protein content was determined using Pierce BCA protein assay kit (Thermo Fisher) according to the manufacturer’s recommendation. .. For immunoblots, equal amounts of each protein sample were separated in an SDS-polyacrylamide gel followed by conventional blotting and a Marker:Article Title: LAT1 enables T cell activation under inflammatory conditions. Article Snippet: The aim of this study was to assess the L-type amino acid transporter-1 (LAT1) as a possible therapeutic target for rheumatoid arthritis (RA).. Synovial LAT1 expression in RA was monitored by immunohistochemistry and transcriptomic datasets.. The contribution of LAT1 to gene expression and immune synapse formation was assessed by RNA-sequencing and total internal reflection fluorescent (TIRF) microscopy, respectively. Article Title: LAT1 enables T cell activation under inflammatory conditions: a new therapeutic target for rheumatoid arthritis Article Snippet: Lysates were clarified by centrifugation, and the protein content was determined using Pierce BCA protein assay kit (Thermo Fisher) according to the manufacturer’s recommendation. .. For immunoblots, equal amounts of each protein sample were separated on a NuPAGE Novex 4-12% Bis-Tris gel followed by conventional blotting and a Article Title: Inhibition of the SEC61 translocon by mycolactone induces a protective autophagic response controlled by EIF2S1-dependent translation that does not require ULK1 activity Article Snippet: Supernatants (soluble) were removed and protein content was quantified by Pierce BCA assay (Thermo Fisher Scientific, 23227) before normalization to the lowest concentration. .. Pellets (insoluble) were dissolved in “gel sample buffer” (50 mM Tris, pH 6.8, 10% glycerol [Thermo Fisher Scientific, G/0650/17], 2.5% β-mercaptoethanol, 2% sodium dodecyl sulfate [Thermo Fisher Scientific, 1281–1680], 0.01% bromophenol blue [BDH Chemicals, 44305]) and sonicated for 30 s. Alternatively, whole cell lysates were prepared directly in gel sample buffer and sonicated for 30 s. Lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis alongside a Article Title: Inhibition of Sec61-dependent translocation by mycolactone uncouples the integrated stress response from ER stress, driving cytotoxicity via translational activation of ATF4 Article Snippet: For western blots, cells were lysed either in RIPA buffer (Sigma) supplemented with protease inhibitors (Sigma) and phosphatase inhibitors (1 mM sodium pyrophosphate, 1 mM PMSF, 5 mM sodium fluoride and 1.75 mM β-glycerophosphate) or gel sample buffer followed by sonication for 30 s. Following RIPA lysis, lysates were clarified by centrifugation, and the protein content was determined using Pierce BCA protein assay kit (Thermo Fisher) according to the manufacturer’s recommendation. .. For immunoblots, equal amounts of each protein sample were separated in an SDS-polyacrylamide gel followed by conventional blotting and a Molecular Weight:Article Title: LAT1 enables T cell activation under inflammatory conditions. Article Snippet: The aim of this study was to assess the L-type amino acid transporter-1 (LAT1) as a possible therapeutic target for rheumatoid arthritis (RA).. Synovial LAT1 expression in RA was monitored by immunohistochemistry and transcriptomic datasets.. The contribution of LAT1 to gene expression and immune synapse formation was assessed by RNA-sequencing and total internal reflection fluorescent (TIRF) microscopy, respectively. Article Title: LAT1 enables T cell activation under inflammatory conditions: a new therapeutic target for rheumatoid arthritis Article Snippet: Lysates were clarified by centrifugation, and the protein content was determined using Pierce BCA protein assay kit (Thermo Fisher) according to the manufacturer’s recommendation. .. For immunoblots, equal amounts of each protein sample were separated on a NuPAGE Novex 4-12% Bis-Tris gel followed by conventional blotting and a Article Title: Inhibition of Sec61-dependent translocation by mycolactone uncouples the integrated stress response from ER stress, driving cytotoxicity via translational activation of ATF4 Article Snippet: For western blots, cells were lysed either in RIPA buffer (Sigma) supplemented with protease inhibitors (Sigma) and phosphatase inhibitors (1 mM sodium pyrophosphate, 1 mM PMSF, 5 mM sodium fluoride and 1.75 mM β-glycerophosphate) or gel sample buffer followed by sonication for 30 s. Following RIPA lysis, lysates were clarified by centrifugation, and the protein content was determined using Pierce BCA protein assay kit (Thermo Fisher) according to the manufacturer’s recommendation. .. For immunoblots, equal amounts of each protein sample were separated in an SDS-polyacrylamide gel followed by conventional blotting and a Sonication:Article Title: Inhibition of the SEC61 translocon by mycolactone induces a protective autophagic response controlled by EIF2S1-dependent translation that does not require ULK1 activity Article Snippet: Supernatants (soluble) were removed and protein content was quantified by Pierce BCA assay (Thermo Fisher Scientific, 23227) before normalization to the lowest concentration. .. Pellets (insoluble) were dissolved in “gel sample buffer” (50 mM Tris, pH 6.8, 10% glycerol [Thermo Fisher Scientific, G/0650/17], 2.5% β-mercaptoethanol, 2% sodium dodecyl sulfate [Thermo Fisher Scientific, 1281–1680], 0.01% bromophenol blue [BDH Chemicals, 44305]) and sonicated for 30 s. Alternatively, whole cell lysates were prepared directly in gel sample buffer and sonicated for 30 s. Lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis alongside a Nucleic Acid Electrophoresis:Article Title: Inhibition of the SEC61 translocon by mycolactone induces a protective autophagic response controlled by EIF2S1-dependent translation that does not require ULK1 activity Article Snippet: Supernatants (soluble) were removed and protein content was quantified by Pierce BCA assay (Thermo Fisher Scientific, 23227) before normalization to the lowest concentration. .. Pellets (insoluble) were dissolved in “gel sample buffer” (50 mM Tris, pH 6.8, 10% glycerol [Thermo Fisher Scientific, G/0650/17], 2.5% β-mercaptoethanol, 2% sodium dodecyl sulfate [Thermo Fisher Scientific, 1281–1680], 0.01% bromophenol blue [BDH Chemicals, 44305]) and sonicated for 30 s. Alternatively, whole cell lysates were prepared directly in gel sample buffer and sonicated for 30 s. Lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis alongside a |